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nsc 34 cells undifferentiated neuro 2a  (ATCC)


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    Structured Review

    ATCC nsc 34 cells undifferentiated neuro 2a
    Nsc 34 Cells Undifferentiated Neuro 2a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nsc+34+cells+undifferentiated+neuro+2a/Neuro-2a/pm41713559-50-8-15
    Average 99 stars, based on 4218 article reviews
    nsc 34 cells undifferentiated neuro 2a - by Bioz Stars, 2026-09
    99/100 stars

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    Cell Culture:

    Article Title: Differential inclusion formation of an aggregation-prone protein reveals differences in the proteostasis capacity of neuronal cell lines.
    Article Snippet: Plasmids encoding wild-type (WT) and double mutant (DM; R188Q, R261Q) Fluc with an N-terminal EGFP tag (FlucWT-EGFP and FlucDM-EGFP) [36] were gifted by Professor Ulrich Hartl (Max Planck Institute of Biochemistry, Munich, Germany) and were cloned into pcDNA4/TO/myc/hisA for mammalian expression by GenScript (Piscataway, NJ, .. Cell culture, transfection and treatment of Neuro-2a and NSC-34 cells Undifferentiated Neuro-2a and NSC-34 cells (American Type Culture Collection, Manassas, VA, USA) were cultured in DMEM/F-12 supplemented with 2.5 mM Lglutamine (Gibco, Carlsbad, CA, USA) and 10% (v/v) foetal calf serum (FCS) (Gibco) at 37°C under 5% CO2/95% air in a Heracell 150i CO2 incubator (Thermo Fisher Scientific, Glen Burnie, MD, USA) and were routinely tested for mycoplasma contamination (~ every 6 months). .. For transient transfections, 1.3 × 105 cells/mL were seeded in CELLSTAR® 6-well plates (Greiner Bio-One, Frickenhausen, Germany) or 8-well chamber μ-Slides (Ibidi, Martinsried, Germany).

    Transfection:

    Article Title: Differential inclusion formation of an aggregation-prone protein reveals differences in the proteostasis capacity of neuronal cell lines.
    Article Snippet: Plasmids encoding wild-type (WT) and double mutant (DM; R188Q, R261Q) Fluc with an N-terminal EGFP tag (FlucWT-EGFP and FlucDM-EGFP) [36] were gifted by Professor Ulrich Hartl (Max Planck Institute of Biochemistry, Munich, Germany) and were cloned into pcDNA4/TO/myc/hisA for mammalian expression by GenScript (Piscataway, NJ, .. Cell culture, transfection and treatment of Neuro-2a and NSC-34 cells Undifferentiated Neuro-2a and NSC-34 cells (American Type Culture Collection, Manassas, VA, USA) were cultured in DMEM/F-12 supplemented with 2.5 mM Lglutamine (Gibco, Carlsbad, CA, USA) and 10% (v/v) foetal calf serum (FCS) (Gibco) at 37°C under 5% CO2/95% air in a Heracell 150i CO2 incubator (Thermo Fisher Scientific, Glen Burnie, MD, USA) and were routinely tested for mycoplasma contamination (~ every 6 months). .. For transient transfections, 1.3 × 105 cells/mL were seeded in CELLSTAR® 6-well plates (Greiner Bio-One, Frickenhausen, Germany) or 8-well chamber μ-Slides (Ibidi, Martinsried, Germany).



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    99
    ATCC nsc 34 cells undifferentiated neuro 2a
    Nsc 34 Cells Undifferentiated Neuro 2a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nsc+34+cells+undifferentiated+neuro+2a/Neuro-2a/pm41713559-50-8-15
    Average 99 stars, based on 1 article reviews
    nsc 34 cells undifferentiated neuro 2a - by Bioz Stars, 2026-09
    99/100 stars
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